4BZ4
CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9794, 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.840, 113.170, 81.490 |
| Unit cell angles | 90.00, 104.51, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.15906 |
| Rwork | 0.158 |
| R-free | 0.18809 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.010 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.430 |
| Number of reflections | 168264 | |
| <I/σ(I)> | 15.3 | 2.8 |
| Completeness [%] | 98.6 | 97 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 11.5-14% PEG 8K, 0.1 BISTRIS PH 7 |
