4BZ2

Structure of dengue virus EDIII in complex with Fab 2D73

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-16
Detector	ADSC QUANTUM 315r
Spacegroup name	P 43 21 2
Unit cell lengths	108.655, 108.655, 108.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.592 - 2.030
R-factor	0.2062
Rwork	0.205
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r29
RMSD bond length	0.015
RMSD bond angle	0.700
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.590	2.100
High resolution limit [Å]	2.030	2.030
Rmerge	0.100
Number of reflections	42571
<I/σ(I)>	16.59	2.03
Completeness [%]	100.0	100
Redundancy	19.7	15.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2 M SODIUM ACETATE PH 5.0, 23% (W/V) PEG 3350