4BY5
Crystal structure of Drosophila Frq2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-05 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.984, 131.192, 56.710 |
Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
Resolution | 42.930 - 2.220 |
R-factor | 0.21366 |
Rwork | 0.211 |
R-free | 0.27052 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g8i |
RMSD bond length | 0.016 |
RMSD bond angle | 1.792 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.970 | 2.340 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.080 | 0.470 |
Number of reflections | 40192 | |
<I/σ(I)> | 12.2 | 2.6 |
Completeness [%] | 99.4 | 96.5 |
Redundancy | 3.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 21% PEG 4000, 0.1 M CACL2, 0.1 M HEPES PH 7.5 |