4BY4
Crystal structure of Drosophila Frq2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | ADSC QUANTUM 351R |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.560, 57.570, 128.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.460 - 2.300 |
| R-factor | 0.21811 |
| Rwork | 0.215 |
| R-free | 0.27496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.525 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.460 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.160 | 0.600 |
| Number of reflections | 18042 | |
| <I/σ(I)> | 7.9 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 26% PEG 4000, 0.1 M CALCIUM CHLORIDE, 0.1 M HEPES PH 7.5 PLUS TRITON X-114 |
