4BXW
Crystal Structure of the Prothrombinase Complex from the Venom of Pseudonaja Textilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-21 |
| Detector | ADSC CCD |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 179.310, 179.310, 179.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.200 - 2.710 |
| R-factor | 0.15917 |
| Rwork | 0.155 |
| R-free | 0.23308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL BASED ON PDB ENTRY 1HCG |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.669 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.200 | 2.850 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.120 | 0.690 |
| Number of reflections | 26001 | |
| <I/σ(I)> | 10.3 | 2.4 |
| Completeness [%] | 1.0 | 99.3 |
| Redundancy | 5.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 45MM MES PH 5.5 AND 5.6% PVP K12 |
