4BXS
Crystal Structure of the Prothrombinase Complex from the Venom of Pseudonaja Textilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 115.310, 115.310, 429.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.810 - 3.320 |
| R-factor | 0.30124 |
| Rwork | 0.298 |
| R-free | 0.36793 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNPUBLISHED FV STRUCTURE AND PDB ENTRY 1KIG |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.614 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.590 | 3.500 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Rmerge | 0.150 | 1.000 |
| Number of reflections | 43913 | |
| <I/σ(I)> | 7.8 | 1.5 |
| Completeness [%] | 99.4 | 95.1 |
| Redundancy | 6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M MORPHEUS BUFFER 3, 20% MORPHEUS P20K_P550MME, 50 MM MORPHEUS CARBOXYLIC ACIDS, pH 8 |
