4BX0
Crystal Structure of a Monomeric Variant of murine Chronophin (Pyridoxal Phosphate phosphatase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.130, 91.820, 39.190 |
| Unit cell angles | 90.00, 98.28, 90.00 |
Refinement procedure
| Resolution | 28.404 - 1.750 |
| R-factor | 0.1936 |
| Rwork | 0.191 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oyc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.006 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.610 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 25533 | |
| <I/σ(I)> | 10.2 | 2.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M MES PH 6.5, 25% PEG-MME 550 |
