4BWX
Structure of Neurospora crassa PAN3 pseudokinase mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 |
| Unit cell lengths | 90.312, 90.312, 230.609 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.156 - 2.850 |
| R-factor | 0.2126 |
| Rwork | 0.211 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bwk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.401 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.410 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.080 | 0.730 |
| Number of reflections | 24793 | |
| <I/σ(I)> | 13.9 | 2.2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M HEPES PH 7.0, 15% PEG MONOMETHYL ETHER 5000 MME, 0.1 M POTASSIUM CHLORIDE |
