4BWN
NEMO CC2-LZ DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 287 |
| Detector technology | PIXEL |
| Collection date | 2011-05-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 |
| Unit cell lengths | 70.353, 70.353, 78.981 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.180 - 2.270 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v4h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER (RM) |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.180 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.100 | |
| Number of reflections | 9976 | |
| <I/σ(I)> | 7.47 | 4.34 |
| Completeness [%] | 96.8 | 87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM LITHIUM CHLORIDE, 10MM HEPES PH 7,5 AND 25% PEG 6000 |
