4BWM
KlenTaq mutant in complex with a RNA/DNA hybrid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 143.192, 86.282, 63.094 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.985 - 1.749 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.20760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rtv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1411)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 1.120 |
| Number of reflections | 79729 | |
| <I/σ(I)> | 13.73 | 1.7 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 6.59 | 6.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | HANGING DROP. 100MM TRIS PH8.5, 200MM MG-FORMATE, 20% PEG 8K MICROSEEDED |
