4BWD
Human short coiled coil protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-07 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.980, 114.750, 93.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.905 - 2.702 |
| R-factor | 0.2209 |
| Rwork | 0.219 |
| R-free | 0.26010 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.580 |
| Number of reflections | 10743 | |
| <I/σ(I)> | 17.3 | 3.7 |
| Completeness [%] | 99.5 | 96.4 |
| Redundancy | 6.5 | 6.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.01 M COBALT CHLORIDE 0.1 M SODIUM ACETATE PH 4.63, 0.7 M 1,6 HEXANEDIOL |
