4BUM
Crystal structure of the Voltage Dependant Anion Channel 2 from zebrafish.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-16 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 72.470, 72.470, 177.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.286 - 2.801 |
| R-factor | 0.2492 |
| Rwork | 0.245 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3emn |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.871 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.290 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.080 |
| Number of reflections | 13686 |
| <I/σ(I)> | 13 |
| Completeness [%] | 98.0 |
| Redundancy | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRISHCL (PH=8.0), 100 MM KCL, AND PEG 2000 IN A RANGE OF 19-24%, 0.011% N-UNDECYL-BETA-D-THIOMALTOPYRANOSIDE |
