4BUJ
Crystal structure of the S. cerevisiae Ski2-3-8 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 183.375, 200.415, 340.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.124 - 3.700 |
| R-factor | 0.2325 |
| Rwork | 0.231 |
| R-free | 0.26490 |
| Structure solution method | SAD |
| Starting model (for MR) | PDB ENTRIES 1S4U 4a4z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.805 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.000 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.140 | 0.910 |
| Number of reflections | 132568 | |
| <I/σ(I)> | 5.8 | 1.4 |
| Completeness [%] | 99.1 | 99.2 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.88 M AMMONIUM SULFATE, 100 MM BISTRIS PH 6.0 |
