4BU2
60S ribosomal protein L27A histidine hydroxylase (MINA53) in complex with Ni(II) and 2-oxoglutarate (2OG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 184.632, 184.632, 184.632 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.690 - 2.780 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.23300 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.690 | 2.930 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.210 | 1.470 |
| Number of reflections | 27690 | |
| <I/σ(I)> | 20.4 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 40.7 | 42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M BIS-TRIS PROPANE PH 6.5, 19-22% (W/V) PEG 3350, 0.2M AMMONIUM SULPHATE, 0.005M NICL2, TEMPERATURE 293K. VAPOR DIFFUSION, SITTING DROP |
