4BU1

Crystal structure of Rad4 BRCT1,2 in complex with a Crb2 phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 21 21 21
Unit cell lengths	65.439, 76.844, 92.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.540 - 2.100
R-factor	0.2397
Rwork	0.237
R-free	0.28790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bmc
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	CrystalClear (2.0)
Data scaling software	DTSCALEAVERAGE
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.010	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.110	0.360
Number of reflections	27555
<I/σ(I)>	7.9	1.8
Completeness [%]	99.2	93.7
Redundancy	4.61	2.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				100 MM NAK PHOSPHATE, 200 MM NACL, 40% W/V PEG 200