4BSZ
Crystal Structure of the Yeast Ribosomal Protein Rps3 in Complex with its Chaperone Yar1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 104.830, 104.830, 95.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.574 - 2.842 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2J00 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.915 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.570 | 3.010 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.070 | 0.790 |
| Number of reflections | 12331 | |
| <I/σ(I)> | 19.85 | 2.86 |
| Completeness [%] | 93.9 | 94 |
| Redundancy | 8.19130646 | 8.41671018 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MES PH 6.0, 200 MM LITHIUM SULPHATE, 20% PEG 4000 |
