4BSM
Crystal structure of the Nuclear Export Receptor CRM1 (exportin-1) lacking the C-terminal helical extension at 4.5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-05 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 148.640, 248.330, 107.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.589 - 4.500 |
| R-factor | 0.2329 |
| Rwork | 0.229 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gb8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.741 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1056)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.590 | 4.740 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.150 | 0.600 |
| Number of reflections | 12112 | |
| <I/σ(I)> | 7.1 | 2.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | BY MIXING EQUAL VOLUMES OF PROTEIN (8-10 MG/ML) AND RESERVOIR SOLUTION 0.5 M DISODIUM TARTRATE, 50 MM TRIS PH 8.0 |
