4BRY
The Idas:Geminin heterodimeric parallel coiled-coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 117.799, 117.799, 103.462 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.946 - 2.890 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uii |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.296 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.950 | 3.050 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.130 | 0.840 |
| Number of reflections | 8415 | |
| <I/σ(I)> | 9.8 | 2.3 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 6.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M SPG PH 7.5, 10 % ISOPROPANOL |
