4BRW
Crystal structure of the yeast Dhh1-Pat1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 105.635, 105.635, 122.068 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.474 - 2.795 |
R-factor | 0.2063 |
Rwork | 0.204 |
R-free | 0.24710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s2m |
RMSD bond length | 0.003 |
RMSD bond angle | 0.743 |
Data reduction software | XDS |
Data scaling software | XDS (CORRECT) |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.880 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.080 | 0.760 |
Number of reflections | 17668 | |
<I/σ(I)> | 18.3 | 2.8 |
Completeness [%] | 99.6 | 97.1 |
Redundancy | 8.6 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 50 MM TRIS PH 8.0, 4% MPD, 200 MM NACL, 25% PEG 400 |