4BRS
Structure of wild type PhaZ7 PHB depolymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-09 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.400, 199.700, 44.200 |
Unit cell angles | 90.00, 114.20, 90.00 |
Refinement procedure
Resolution | 21.923 - 1.600 |
R-factor | 0.1336 |
Rwork | 0.132 |
R-free | 0.17040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vtv |
RMSD bond length | 0.012 |
RMSD bond angle | 1.282 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.920 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.020 | 0.120 |
Number of reflections | 81064 | |
<I/σ(I)> | 27.39 | 6.63 |
Completeness [%] | 94.3 | 81.2 |
Redundancy | 2.5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 0.1 M NAOAC (PH 5.0), 0.2 M MGCL2 AND 18 % W/V PEG 6000 |