4BRR
Crystal structure of the integral membrane diacylglycerol kinase - delta 7.79
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-06 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.060, 91.500, 143.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.533 - 2.440 |
R-factor | 0.2195 |
Rwork | 0.218 |
R-free | 0.25220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ze3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.688 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.060 | 2.500 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.080 | 1.030 |
Number of reflections | 37160 | |
<I/σ(I)> | 8.6 | 2.1 |
Completeness [%] | 99.0 | 99.4 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 4-6 %(V/V) 2-METHYL-2, 4-PENTANEDIOL (MPD), 0.1 M SODIUM CHLORIDE, 60 MM MAGNESIUM ACETATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE) METHOD AT 4 DEGREES CELCIUS WITH THE 7.9 MONOACYLGLYCEROL (7.9 MAG) AS THE HOSTING LIPID. |