4BRO
Legionella pneumophila NTPDase1 crystal form IV (part-open)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 155.991, 155.991, 79.222 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.170 - 1.990 |
| R-factor | 0.21509 |
| Rwork | 0.215 |
| R-free | 0.24069 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.526 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.140 |
| Number of reflections | 74335 |
| <I/σ(I)> | 6.9 |
| Completeness [%] | 99.1 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100MM HEPES PH 7.5, 1.4 M (NH4)2SO4 |
