4BR7
Legionella pneumophila NTPDase1 crystal form I, open, AMPNP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 102.853, 102.853, 74.779 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.610 - 1.800 |
| R-factor | 0.16377 |
| Rwork | 0.163 |
| R-free | 0.20941 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.978 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.060 |
| Number of reflections | 42613 |
| <I/σ(I)> | 12.6 |
| Completeness [%] | 100.0 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 30% PEP 629 (PENTAERYTHRITOL PROPOXYLATE 17/8 PO/OH), 100MM NAHEPES PH 7.7, 50MM MGCL2 |
