4BQQ
Protein crystal structure of the N-terminal and recombinase domains of the Streptomyces temperate phage serine recombinase, fC31 integrase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 |
| Unit cell lengths | 96.040, 96.040, 117.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.040 - 2.150 |
| R-factor | 0.21963 |
| Rwork | 0.218 |
| R-free | 0.24427 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.159 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.040 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.050 | 0.650 |
| Number of reflections | 56465 | |
| <I/σ(I)> | 25.1 | 2.6 |
| Completeness [%] | 98.3 | 97.3 |
| Redundancy | 4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M SODIUM CITRATE PH5.5, 1.55M DI AMMONIUM HYDROGEN PHOSPHATE |
