4BQK
rice importin_alpha : VirD2NLS complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2012-01-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.803, 140.948, 72.632 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.724 - 1.997 |
| R-factor | 0.1972 |
| Rwork | 0.196 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b8o |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.720 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.140 | 0.600 |
| Number of reflections | 85023 | |
| <I/σ(I)> | 6.1 | 2.1 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 16 % PEG3350, 0.1 M BIS-TRISPROPANE PH 6.5, 0.2 M NAF, 0.2 M NDSB-221 |
