4BPW
Crystal structure of human primase bound to UTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.394, 69.438, 127.087 |
| Unit cell angles | 90.00, 104.90, 90.00 |
Refinement procedure
| Resolution | 46.784 - 3.003 |
| R-factor | 0.2172 |
| Rwork | 0.215 |
| R-free | 0.25340 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.924 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.870 | 3.130 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.060 | 1.500 |
| Number of reflections | 39304 | |
| <I/σ(I)> | 18.5 | 1.2 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM TRIS-HCL/BICINE PH 8.5, 20% GLYCEROL, 10% PEG4000 AND 20MM EACH OF AN ALCOHOL MIX COMPRISING 1,6-HEXANEDIOL, 1-BUTANOL, 1,2-PROPANEDIOL, 2-PROPANOL, 1,4-BUTANEDIOL AND 1,3-PROPANEDIOL. |
