4BOW
Crystal structure of LamA_E269S from Z. galactanivorans in complex with laminaritriose and laminaritetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.459, 76.495, 142.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.440 - 1.350 |
| R-factor | 0.13184 |
| Rwork | 0.130 |
| R-free | 0.16666 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bq1 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.324 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.440 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.040 | 0.550 |
| Number of reflections | 107451 | |
| <I/σ(I)> | 16.3 | 2.3 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 100 MM MIB PH 4.0, 19% PEG1500, HANGING DROP AT 20 DEGREES CELSIUS MIXING 2 MICROL OF PROTEIN (13.7 MG/ML SUPPLEMENTED WITH 5 MM PURIFIED LAMINARIHEXAOSE) WITH 1 MICROL OF RESERVOIR SOLUTION |
