4BOU
Structure of OTUD3 OTU domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 36.196, 36.196, 211.051 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.347 - 1.550 |
| R-factor | 0.1771 |
| Rwork | 0.175 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pfy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.916 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.210 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.040 | 0.480 |
| Number of reflections | 23429 | |
| <I/σ(I)> | 14.9 | 2.1 |
| Completeness [%] | 98.9 | 99.1 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% PEG 4K, 0.1 M KCL, 0.01 M MGCL2, 50 MM MES PH 6.0 |
