4BNE
Pacsin2 Interacts with Membranes and Actin-Filaments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.060, 105.500, 125.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.447 - 2.570 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.22220 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.515 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.450 | 2.660 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 41812 | |
| <I/σ(I)> | 15.12 | 2.25 |
| Completeness [%] | 96.0 | 77.5 |
| Redundancy | 4.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PEG2000MME, AMMONIUM SULFATE, HEPES PH 8.0 |
