4BMP
Crystal Structure of Bacillus cereus Ribonucleotide Reductase di- iron NrdF in Complex with NrdI (2.1 A resolution)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.330, 125.030, 142.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.590 - 2.100 |
| R-factor | 0.1798 |
| Rwork | 0.177 |
| R-free | 0.23342 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmo |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.035 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.500 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 30834 | |
| <I/σ(I)> | 8.6 | 2.8 |
| Completeness [%] | 98.5 | 98.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10 % (W/V) PEG 8000, 0.2 M MAGNESIUM CHLORIDE AND 0.1 M TRIS, PH 7.0 |
