4BMO
Crystal Structure of Bacillus cereus Ribonucleotide Reductase di- iron NrdF in Complex with NrdI (1.8 A resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.490, 124.670, 141.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.750 - 1.810 |
R-factor | 0.1689 |
Rwork | 0.167 |
R-free | 0.20942 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x2o |
RMSD bond length | 0.020 |
RMSD bond angle | 1.903 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.700 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.450 |
Number of reflections | 47107 | |
<I/σ(I)> | 11.3 | 2.9 |
Completeness [%] | 98.2 | 99.3 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 2 M SODIUM CHLORIDE AND 0.1 M SODIUM CITRATE, PH 6.0 |