4BMK
Serine Palmitoyltransferase K265A from S. paucimobilis with bound PLP- Myriocin Aldimine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-06 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.910, 98.710, 133.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.140 - 1.620 |
| R-factor | 0.18998 |
| Rwork | 0.189 |
| R-free | 0.21086 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jg2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.440 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.140 | 1.660 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 103044 | |
| <I/σ(I)> | 10.8 | 2.3 |
| Completeness [%] | 98.9 | 99.7 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 32% PEG MME 2000, 0.1M HEPES PH 7.5 |
