4BMJ
Structure of the UBZ1and2 tandem of the ubiquitin-binding adaptor protein TAX1BP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2007-12-13 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 118.878, 118.878, 327.345 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 103.140 - 2.750 |
| R-factor | 0.21673 |
| Rwork | 0.215 |
| R-free | 0.25459 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.346 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.500 | 2.730 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.100 | 0.790 |
| Number of reflections | 43605 | |
| <I/σ(I)> | 6.38 | 0.96 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.1 | 10.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5, 1 M LISO4 |
