4BMG
Crystal structure of hexameric HBc149 Y132A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2013-02-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.790, 67.750, 86.610 |
| Unit cell angles | 68.98, 70.75, 84.51 |
Refinement procedure
| Resolution | 44.526 - 3.000 |
| R-factor | 0.2555 |
| Rwork | 0.252 |
| R-free | 0.31730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kxs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.450 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.690 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.030 | 0.430 |
| Number of reflections | 24745 | |
| <I/σ(I)> | 17.2 | 1.8 |
| Completeness [%] | 95.3 | 94 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 293 | 100 MM CITRATE PH 5.0, 15 % (V/V) ISO-PROPANOL, 1% (W/V) PEG 10 000, 293 K |
