4BM7
Crystal Structure of IgG Fc F241A mutant with native glycosylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-05 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.868, 73.375, 135.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.920 - 1.950 |
| R-factor | 0.19974 |
| Rwork | 0.198 |
| R-free | 0.23428 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ave |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.940 | 1.950 |
| Rmerge | 0.090 | 0.750 |
| Number of reflections | 36211 | |
| <I/σ(I)> | 16.4 | 2 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 28% POLYETHYLENE GLYCOL MONOMETHYL ETHER 2,000 IN 0.1M BIS-TRIS BUFFER AT PH 6.5 |
