4BLQ
P4 PROTEIN FROM BACTERIOPHAGE PHI8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 138.940, 159.570, 100.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.790 |
| R-factor | 0.215 |
| Rwork | 0.214 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.500 | 2.850 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.130 | 0.880 |
| Number of reflections | 56752 | |
| <I/σ(I)> | 14.6 | 3.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.6 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS PH 8.0 AND 18% PEG 1000 |
