4BL9

Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-02
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 1
Unit cell lengths	93.430, 103.280, 111.510
Unit cell angles	63.67, 81.13, 76.03

Refinement procedure

Resolution	29.476 - 2.800
R-factor	0.2011
Rwork	0.200
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4BL8 AND 1OMP
RMSD bond length	0.006
RMSD bond angle	0.850
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.130
Number of reflections	87954
<I/σ(I)>	12.9	2
Completeness [%]	98.6	98.1
Redundancy	7.7	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	277	PROTEIN (6.5 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT, 1 MM MALTOSE) WAS CRYSTALLISED AT 4C BY HANGING DROP VAPOUR DIFFUSION WITH 0.08 M NA-CACODYLATE (PH 6.6), 20% (V/V) GLYCEROL, 160 MM CA(OAC)2 AND 9% (V/V) PEG 8000