4BKW
Crystal structure of the C-terminal region of human ZFYVE9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 113.751, 113.751, 155.098 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.700 - 2.530 |
R-factor | 0.2056 |
Rwork | 0.203 |
R-free | 0.24730 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | CCP4 |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.990 | 2.670 |
High resolution limit [Å] | 2.530 | 2.530 |
Rmerge | 0.090 | 0.560 |
Number of reflections | 20314 | |
<I/σ(I)> | 14.2 | 3.8 |
Completeness [%] | 99.8 | 100 |
Redundancy | 8.5 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1M BIS-TRIS PH 6.5, 0.15M MAGNESIUM CHLORIDE, 30% PEG 3350 |