4BKN
Human Dihydropyrimidinase-related protein 3 (DPYSL3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-31 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.230, 96.650, 119.270 |
| Unit cell angles | 90.00, 137.26, 90.00 |
Refinement procedure
| Resolution | 42.890 - 2.100 |
| R-factor | 0.19946 |
| Rwork | 0.197 |
| R-free | 0.23813 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vm8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.302 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.850 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.180 | 0.450 |
| Number of reflections | 60144 | |
| <I/σ(I)> | 5.6 | 2.9 |
| Completeness [%] | 93.0 | 94.2 |
| Redundancy | 5.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2M KSCN; 0.1M BISTRISPROPANE PH 8.5; 20% PEG 3350 10% ETHYLENE GLYCOL |
