4BK4

crystal structure of the human EphA4 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Spacegroup name	P 32 2 1
Unit cell lengths	166.890, 166.890, 192.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.180 - 3.650
R-factor	0.3513
Rwork	0.349
R-free	0.38580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x10
RMSD bond length	0.007
RMSD bond angle	0.850
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.000	3.700
High resolution limit [Å]	3.650	3.650
Rmerge	0.190
Number of reflections	34863
<I/σ(I)>	14.7	0.48
Completeness [%]	99.9	100
Redundancy	20	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1.8 M AMMONIUM PHOSPHATE, 100 MM HEPES PH 7.4, ADDITIVE = EPHRINB2 IN A 1:1 MOLAR RATIO. WE SAW NO SIGN OF EPHRINB2 IN THE CRYSTAL STRUCTURE. THE USUAL BINDING SITE ON HEPHA4 WAS OCCUPIED BY AN EPHA4-EPHA4 CRYSTAL CONTACT.
1				1.8 M AMMONIUM PHOSPHATE, 100 MM HEPES PH 7.4, ADDITIVE = EPHRINB2 IN A 1:1 MOLAR RATIO. WE SAW NO SIGN OF EPHRINB2 IN THE CRYSTAL STRUCTURE. THE USUAL BINDING SITE ON HEPHA4 WAS OCCUPIED BY AN EPHA4-EPHA4 CRYSTAL CONTACT.