4BJS
Crystal structure of the Rif1 C-terminal domain (Rif1-CTD) from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.789, 34.842, 46.214 |
| Unit cell angles | 87.41, 79.89, 82.31 |
Refinement procedure
| Resolution | 17.260 - 1.940 |
| R-factor | 0.1721 |
| Rwork | 0.171 |
| R-free | 0.19630 |
| Structure solution method | DIRECT METHODS |
| Starting model (for MR) | 14-MER POLY-ALA IDEALIZED ALPHA-HELIX |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.890 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | Arcimboldo |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 1.950 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.080 | 0.360 |
| Number of reflections | 15001 | |
| <I/σ(I)> | 11.2 | 2.1 |
| Completeness [%] | 96.4 | 73.6 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (W/V) PEG 3000, 200 MM NACL, 100 HEPES/NAOH PH 7.5. PROTEIN WAS TREATED WITH 0.003% TRYPSIN IMMEDIATELY PRIOR TO CRYSTALLIZATION. |
