4BJJ
Sfc1-Sfc7 dimerization module
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 85.245, 85.245, 154.108 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.290 - 2.400 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22360 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.237 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.620 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.110 | 0.890 |
| Number of reflections | 13629 | |
| <I/σ(I)> | 14.5 | 2.1 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 12.1 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.4 M AMSO4, 0.1 M HEPES PH 7, 0.1 M KCL |
