4BJ6
Crystal structure Rif2 in complex with the C-terminal domain of Rap1 (Rap1-RCT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.250, 112.680, 140.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.790 - 3.260 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.19530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4BJ1 AND 3OWT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 3.270 |
| High resolution limit [Å] | 3.260 | 3.260 |
| Rmerge | 0.050 | 0.500 |
| Number of reflections | 27519 | |
| <I/σ(I)> | 22.1 | 3.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22-25% PEG 6000, 100 MM TRIS/HCL PH 8.0, 500 MM LI2SO4 |
