4BJ4
Structure of Pseudomonas aeruginosa amidase Ampdh2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.759, 100.421, 104.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.267 - 1.722 |
| R-factor | 0.1733 |
| Rwork | 0.172 |
| R-free | 0.20090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wkx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.144 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 54928 | |
| <I/σ(I)> | 28 | 5.2 |
| Completeness [%] | 99.8 | 96.5 |
| Redundancy | 10.3 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 0.2 M POTASSIUM THIOCYANATE, 0.1 M BIS-TRIS PROPANE PH 7.5, 18 % PEG 3350 |
