4BIM
CATALASE 3 FROM NEUROSPORA CRASSA IN TETRAGONAL FORM EXPOSES A MODIFIED TETRAMERIC ORGANIZATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-23 |
| Detector | RIGAKU RAXIS IV PLUS PLUS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 207.508, 207.508, 137.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.300 - 2.950 |
| R-factor | 0.2678 |
| Rwork | 0.255 |
| R-free | 0.30440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ej6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.110 | 0.460 |
| Number of reflections | 62655 | |
| <I/σ(I)> | 9.7 | 2.8 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | MES 100 MM, PH 5.5, 50 % V/V MPD |
