4BIK
Structure of a disulfide locked mutant of Intermedilysin with human CD59
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Collection date | 2012-09-15 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.400, 101.450, 112.880 |
| Unit cell angles | 63.75, 89.95, 90.02 |
Refinement procedure
| Resolution | 45.494 - 3.494 |
| R-factor | 0.2032 |
| Rwork | 0.212 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1S3R AND 2UWR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS (XIA2) |
| Data scaling software | Aimless (XIA2) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.450 | 3.620 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.110 | 0.300 |
| Number of reflections | 13247 | |
| <I/σ(I)> | 7.3 | 2 |
| Completeness [%] | 96.0 | 96.9 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG6000, 0.1 M HEPES PH 7, 0.2 M LICL |
