4BH2
Crystal Structure of the Haemagglutinin from a Transmissible Mutant H5 Influenza Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-10 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | H 3 2 |
Unit cell lengths | 101.620, 101.620, 331.126 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.880 - 2.120 |
R-factor | 0.21442 |
Rwork | 0.213 |
R-free | 0.23517 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.095 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.990 | 2.230 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.080 | 0.630 |
Number of reflections | 36665 | |
<I/σ(I)> | 11.5 | 2.3 |
Completeness [%] | 97.3 | 95.6 |
Redundancy | 4.9 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M HEPES PH 7.0, 25-30% JEFFAMINE ED-2001 |