4BH1

H5 (tyTy) Influenza Virus Haemagglutinin in Complex with Avian Receptor Analogue 3'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-23
Detector	DECTRIS PILATUS 2M
Spacegroup name	P 1 21 1
Unit cell lengths	70.859, 228.288, 71.920
Unit cell angles	90.00, 113.71, 90.00

Refinement procedure

Resolution	62.480 - 2.150
R-factor	0.20611
Rwork	0.205
R-free	0.22411
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.072
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	2.270	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.080	0.580
Number of reflections	110762
<I/σ(I)>	9.7	2.1
Completeness [%]	98.1	94.7
Redundancy	3.5	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				BIS-TRIS PROPANE PH 7.5, 0.05 - 0.15 M K/NAPO4 (PH 7.0), 15-18% PEG 3350