4BGJ
Crystal structure of the phox-homology domain of human sorting nexin 14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-29 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 |
| Unit cell lengths | 88.611, 88.611, 73.082 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.490 - 2.550 |
| R-factor | 0.20625 |
| Rwork | 0.205 |
| R-free | 0.22566 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xtn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.966 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.490 | 2.690 |
| High resolution limit [Å] | 2.440 | 2.550 |
| Rmerge | 0.050 | 1.030 |
| Number of reflections | 9277 | |
| <I/σ(I)> | 22.4 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20%(W/V) PEG 3350, 0.2M SODIUM MALONATE |
