4BGB
Nucleotide-bound closed form of a putative sugar kinase MK0840 from Methanopyrus kandleri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.790, 108.540, 117.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.147 - 1.340 |
| R-factor | 0.1588 |
| Rwork | 0.158 |
| R-free | 0.18280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4BGA CHAIN A |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.482 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.370 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.040 | 0.650 |
| Number of reflections | 154630 | |
| <I/σ(I)> | 21.12 | 2.61 |
| Completeness [%] | 98.3 | 93.2 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.14 M CACL2, 0.07 M SODIUM ACETATE PH 4.6, 10 % ISOPROPANOL AND 28% GLYCEROL |
